PIP5K1 alpha (PIP5K1A) (NM_003557) Human 3' UTR Clone

CAT#: SC215977

3' UTR clone of phosphatidylinositol-4-phosphate 5-kinase type I alpha (PIP5K1A) transcript variant 2 for miRNA target validation



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CNY 5,795.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name PIP5K1 alpha (PIP5K1A) (NM_003557) Human 3' UTR Clone
Vector pMirTarget
ACCN NM_003557
Insert Size 1696 bp
Sequence Data
>SC215977 3’UTR clone of NM_003557
The sequence shown below is from the reference sequence of NM_003557. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
GAAGTTGCAGAGTCAGAGTTCACCCATTAAGCGCAAAGCCTCAGAAGACCTGGAACAAGATTCTGCCAT
CTCTGTGATCCCAAGATGTCAGCCCTTGCCCCAGCAATGCTGAATTTTCTTCTACTTGGTCATCAAAAA
AGGAGTGTAATAGAAGTGAGGGGAGCTGCTCCTCCATCTTCTTCCTGAAGAAGAACCTTCTCTCCTTCC
TCTTCCTCATGAATGGGCCTTAGTGCCTCAGAGAGTTGAGGACCGCAGCATCCCCTCCACTCCAGAGTT
GGGTGGTATGGATTTTCAACTGGCCAACCCTTTGCCTCCACTATTGAATTTTTTTCAGACCCCCATTCT
TCATGCTGGAAATGGGATTGCTGGACTTGGCAGCTTTCTTTCCCCTCGTCTTTGACTAGGAACCGGACT
CTTAATTTCCTCAGGACAGACTAGCTGGCACATTATCCCTACCTTAGTTCTTTCTCTCTGACTCCTGGA
AGAATACTCCTGTAATCTCTGTAAAGGTTTTTGGGGGATAAGGGTGTTTAACCACCTCCCAGCTTTCTT
CTTCTTTTTTTTTTTCTGAAAAAAGGAAAAAGCACACAGCACACAATTTCAAGCCATTTTCAGATCAGA
ACTCCAGAAGTGTTGACAAGATGCCTATTCGTAGAGTTCCCTCAGAAGAGCCATGGTGTTTATGAAGAG
AAGAGTAGTGATTGCTCTGCCAGAAGCAGCTCCTCTTTAAACTCCTCCTCTCTTGATGAATTTCTTAAG
GCTGAAGGAATGAAGAGAGTGGGACATGGGGTAATCTTTATCCCTTTTGTTAAAACAGGAGGCAGCCAT
GGGCTGGGAGATCATAGCCCTTCCTAGGCAGAATCCTGTTCACTGCCAGGCTATAGTAATTATTACTAT
TTTGCAATTTGAAATATATTCTGGTTGTTTTTCTAAATGTGAAGACTTACCAAATGAATTTTAGATCAT
TCTCCAGAGGAGATTTTTTTTGCTCTTCTCATCTTTTCCAACAGTGTTCTCCTGTTTGTGGAGCTAAGG
TAAAGAGGGGACACTTCTGTCTGTTTAACAGACAGTCCATATCTGTGAGGCCAGCAAATATTTTCTTAA
ACTCATGGGGAGACAGCAGATTCTTGCCTTGGTGAGGTCATTGCTGTGCCATATGTCCTACCCCCCTGT
CTTCATGCAGGGAAGTTGGAAATGGGGGCTACATATGCCCTCTCCTCCCCGTCTACAAGAGTTGTGGTT
TTCCATCTGATCCTTCCACTCTTGTCAGGGGAAGAAGGGGGCCTGGTATCTCAGGCAGATTGTTGAATT
CCTGTTCTATCCCTTCTCTATCCCACCCTGCCTTGATAATATGTTAGCCCATACCCCAAATAACTGTCT
ATATTAGACACCCCCAGCCAGTTTCTGGCTGCCTGTCTTTGCTGCCATGTTTTTTACAAGAAGGAAAGA
ATTCTTGCTATTTTTTTTTCATAATTTACTATTTATGATGTATTTAAGTGTTTTATTAAGGACAGAGTT
CTGTTAGGGGTGGGAGGGAATATTTGAGGGAGGGCTGGGTCTTAGGGAAAGGAATGGGGAAGCAACATT
TTTATTAAGTGTTACTATTTGCCTCTACTTTGTATTGTTCAGAAATGGCAAATACAATATAAAAGTGAT
ATATGGTTTTAATGTAATAAACTTTAATGAGTTATTTAGA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_003557.3
Summary Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:18288197, PubMed:19158393, PubMed:20660631). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity).[UniProtKB/Swiss-Prot Function]
Locus ID 8394
MW 63.2
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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