PHD3 (EGLN3) (NM_022073) Human 3' UTR Clone

Specifications

Product Data
Product Name	PHD3 (EGLN3) (NM_022073) Human 3' UTR Clone
Vector	pMirTarget
Synonyms	HIFP4H3; HIFPH3; PHD3
ACCN	NM_022073
Insert Size	1694 bp
Sequence Data	Insert Sequence (showhide) >SC215935 3’UTR clone of NM_022073 The sequence shown below is from the reference sequence of NM_022073. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC AAAACTGAATCTGCCCTCACTGAAGACTGACCGTGCTCTGAAATCTGCTGGCCTTGTTCATTTTAGTAA CGGTTCCTGAATTCTCTTAAATTCTTTGAGATCCAAAGATGGCCTCTTCAGTGACAACAATCTCCCTGC TACTTCTTGCATCCTTCACATCCCTGTCTTGTGTGTGGTACTTCATGTTTTCTTGCCAAGACTGTGTTG ATCTTCAGATACTCTCTTTGCCAGATGAAGTTACTTGCTAACTCCAGAAATTCCTGCAGACATCCTACT CGGCCAGCGGTTTACCTGATAGATTCGGTAATACTATCAAGAGAAGAGCCTAGGAGCACAGCGAGGGAA TGAACCTTACTTGCACTTTATGTATACTTCCTGATTTGAAAGGAGGAGGTTTGAAAAGAAAAAAATGGA GGTGGTAGATGCCACAGAGAGGCATCACGGAAGCCTTAACAGCAGGAAACAGAGAAATTTGTGTCATCT GAACAATTTCCAGATGTTCTTAATCCAGGGCTGTTGGGGTTTCTGGAGAATTATCACAACCTAATGACA TTAATACCTCTAGAAAGGGCTGCTGTCATAGTGAACAATTTATAAGTGTCCCATGGGGCAGACACTCCT TTTTTCCCAGTCCTGCAACCTGGATTTTCTGCCTCAGCCCCATTTTGCTGAAAATAATGACTTTCTGAA TAAAGATGGCAACACAATTTTTTCTCCATTTTCAGTTCTTACCTGGGAACCTAATTCCCCAGAAGCTAA AAAACTAGACATTAGTTGTTTTGGTTGCTTTGTTGGAATGGAATTTAAATTTAAATGAAAGGAAAAATA TATCCCTGGTAGTTTTGTGTTAACCACTGATAACTGTGGAAAGAGCTAGGTCTACTGATATACAATAAA CATGTGTGCATCTTGAACAATTTGAGAGGGGAGGTGGAGTTGGAAATGTGGGTGTTCCTGTTTTTTTTT TTTTTTTTTTTTTAGTTTTCCTTTTTAATGAGCTCACCCTTTAACACAAAAAAAGCAAGGTGATGTATT TTAAAAAAGGAAGTGGAAATAAAAAAATCTCAAAGCTATTTGAGTTCTCGTCTGTCCCTAGCAGTCTTT CTTCAGCTCACTTGGCTCTCTAGATCCACTGTGGTTGGCAGTATGACCAGAATCATGGAATTTGCTAGA ACTGTGGAAGCTTCTACTCCTGCAGTAAGCACAGATCGCACTGCCTCAATAACTTGGTATTGAGCACGT ATTTTGCAAAAGCTACTTTTCCTAGTTTTCAGTATTACTTTCATGTTTTAAAAATCCCTTTAATTTCTT GCTTGAAAATCCCATGAACATTAAAGAGCCAGAAATATTTTCCTTTGTTATGTACGGATATATATATAT ATAGTCTTCCAAGATAGAAGTTTACTTTTTCCTCTTCTGGTTTTGGAAAATTTCCAGATAAGACATGTC ACCATTAATTCTCAACGACTGCTCTATTTTGTTGTACGGTAATAGTTATCACCTTCTAAATTACTATGT AATTTATTCACTTATTATGTTTATTGTCTTGTATCCTTTCTCTGGAGTGTAAGCACAATGAAGACAGGA ATTTTGTATATTTTTAACCAATGCAACATACTCTCAGCACCTAAAATAGTGCCGGGAACATAGTAAGGG CTCAGTAAATACTTGTTGAATAAACTCAGTCTCCTACA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites	SgfI-MluI
OTI Disclaimer	Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq	NM_022073.4
Synonyms	HIFP4H3; HIFPH3; PHD3
Summary	Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function]
Locus ID	112399
MW	65.8

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