Mouse Icam1 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse sICAM-1 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse sICAM-1.
Sensitivity	30pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,8000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Intercellular Adhesion Molecule-1 (ICAM-1) is an inducible transmembrane molecule that plays a role in cell migration, antigen presentation and leukocyte activation. Mouse ICAM-1 is an 80-110 kDa, type I transmembrane glycoprotein that is expressed on a variety of cell types. The molecule is 537 amino acids (aa) in length and contains a 27 aa signal sequence, a 458 aa extracellular region, a 24 aa transmembrane segment, and a 28 aa cytoplasmic domain. The extracellular region contains five Ig-like domains and eleven potential N-linked glycosylation sites, many of which are utilized. The first, N-terminal Ig domain (D1) binds LFA-1, while the third domain (D3) binds Mac-1. Notably, glycosylation on the third domain regulates Mac-1 binding. The cytoplasmic domain, while short, is considered to both transduce intracellular signals (via MAP kinase) and interact with the cell cytoskeleton. Membrane ICAM-1 exists as a dimer and will form multimers via D1 interactions. Monomeric ICAM-1 is competent to bind LFA-1. Soluble, dimeric ICAM-1 does circulate and binds LFA-1 with high avidity. Soluble forms are generated via proteolytic processing, reportedly through MMP-9 and elastase. In mice, there are a number of ICAM-1 alternate splice forms that lack combinations of various Ig-domains. This suggests the possibility of multiple truncated forms of proteolytically-generated circulating ICAM-1. Mature mouse ICAM-1 shares 77%, 53%, 56%, and 52% sequence identity to rat, human, canine and porcine ICAM-1, respectively. Cells known to express ICAM-1 include smooth muscle cells, keratinocytes, endothelial cells, fibroblasts, bronchial epithelial cells , memory T cells, B cells, plasma cells, monocytes, macrophages, CFU-E, CFU-GM, activated eosinophils, neutrophils, Schwann cells, Sertoli cells, melanocytes, and dendritic cells.
Gene Symbol	Icam1

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.