Mouse Cxcl5 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse CXCL5 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse CXCL5.
Sensitivity	15pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Mouse CXCL5, also called LIX [lipopolysaccharide (LPS)-induced CXC chemokine], is a member of the ELR+ group of 7-9 kDa neutrophil and monocyte chemotactic proteins in the CXCL cytokine family. This group contains the ELR/GluLeuArg amino acid (aa) motif immediately N-terminal to the CXC motif. Other ELR+ chemokines also include mouse CXCL1 thru 3 and 15 (or KC, MIP-2, DCIP-1 and lungkine, respectively), plus human CXCL1 thru 3 and 5 thru 8 (or GROα, GROβ, GROγ, ENA-78, GCP-2, NAP-2 and IL-8, respectively). Mouse LIX cDNA encodes 132 aa, including a 40 aa signal peptide and up to a 92 aa mature protein that has a longer C-terminus than other ELR+ cytokines. The first 78 aa of mature LIX shares 63% and 55% aa identity with human CXCL6/GCP-2 and CXCL5/ENA-78, respectively. Its activity is similar but not identical to these human chemokines. In the mouse, CXCL1/KC and CXCL2/MIP-2 expression and function may overlap with that of LIX.
Gene Symbol	Cxcl5

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