Mouse Vcam1 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse VCAM-1 ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse VCAM-1.
Sensitivity	15pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Vascular cell adhesion molecule-1 (VCAM-1), also known as CD106, is a type I membrane glycoprotein belonging to the immunoglobulin superfamily (IgSF) of cell adhesion molecules. By alternative splicing, at least two variants of mouse VCAM-1 have been identified. The type I transmembrane mouse VCAM-1 long isoform is a 110 kDa protein containing a 674 amino acid (aa) residue extracellular domain organized into seven C2-type Ig domains, a 22 aa residue transmembrane domain and a 19 aa residue cytoplasmic domain. The short mouse VCAM-1 isoform is a 45 kDa glycosylphosphatidylinositol (GPI)-anchored protein containing only the first three amino-terminal Ig domains of the transmembrane isoform. Both the transmembrane and the GPI-anchored VCAM-1 isoforms can be released from cells to yield soluble VCAM-1 that is detectable in cell supernates or serum.
Gene Symbol	Vcam1

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