Human Myeloperoxidase (MPO) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Myeloperoxidase Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Myeloperoxidase.
Sensitivity	30 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Reaction of these compounds with macromolecules results in the nitrosylation, chlorination, and oxidation of tyrosine residues, lipids, and cholesterol, and the intermolecular crosslinking of proteins and DNA. MPO is synthesized as a preproprotein that is proteolytically processed to remove a 48 amino acid (aa) signal peptide, a 116 aa propeptide, the C-terminal serine, and a 6 aa internal peptide which generates separate 60 kDa heavy and 12 kDa light chains. Mature human MPO shares 87-88% aa sequence identity with canine, mouse, and rat MPO. It shares 71%, 56%, and 47% aa sequence identity with comparable regions of human eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase, respectively.
Gene Symbol	MPO

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