Human Macrophage Inflammatory Protein 3 alpha (CCL20) ELISA KIT (96 T)

Specifications

Product Data
Description	Human MIP-3α Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	7.8-500 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human MIP-3a.
Sensitivity	4 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	MIP-3α [also known as LARC (liver and activation-regulated chemokine), Exodus-1 or CCL20] is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells (DCs). MIP-3α plays an important role in the regulation of DC trafficking, and recruitment and activation of T cells. The full-length cDNA for MIP-3α encodes a protein of 96 amino acids (aa) that shows relatively low sequence similarity to other human CC chemokines. It shares the highest aa sequence homology with MIP-1β (28% identity). Human MIP-3α demonstrates 64% sequence identity with the mouse orthologue of MIP-3α. MIP-3α contains the four conserved cysteine residues as well as three out of five other aa residues that are highly conserved among CC chemokines. The tyrosine and threonine residues that are typically present between the second and third cysteine residues in other CC chemokines are substituted in MIP-3α with phenylalanine and alanine, respectively. MIP-3α also differs from other CC chemokines in that it maps to human chromosome 2 vs. chromosome 17.
Gene Symbol	CCL20

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.