Human MIF ELISA KIT (96 T)

Specifications

Product Data
Description	Human MIF Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human MIF.
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	One of the first cytokines described, MIF (macrophage migration inhibitory factor) was originally shown to inhibit random macrophage migration, but was later found to be chemotactic. With the discovery that MIF is a non-canonical ligand of chemokine receptors, MIF is now proposed to belong to a group of chemokine-like function (CLF) proteins, or "microchemokines". It is an important mediator of the innate immune response with potential roles in the pathophysiology of inflammatory, autoimmune, and neoplastic disorders. The human MIF gene encodes a 115 amino acid, 12.5 kDa secreted protein. Crystallography studies suggest that MIF exists as a homotrimer, but dimers or monomers are also possible. MIF shares minimal homology with other known cytokines. It exhibits intrinsic tautomerase and oxidoreductase activity, but the physiological importance of MIF as an enzyme remains unclear.
Gene Symbol	MIF

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