Human Tissue Factor (F3) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Coagulation factor III Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	7.8-500 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Coagulation factor III.
Sensitivity	4 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Coagulation Factor III is a 47 kDa transmembrane protein, which consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. In the vessel wall, Coagulation Factor III is constitutively expressed in subendothelial cells, such as vascular smooth muscle cells. Other cell types, such as endothelial cells and monocytes, are also able to express Coagulation Factor III when stimulated with cytokines and growth factors. Coagulation Factor III can be released into the circulation in association with microparticles. Because of its essential function in the coagulation cascade, it is well established that Coagulation Factor III plays an important role in pathological conditions related to hemostasis, such as thrombotic diseases and disseminated intravascular coagulation. In atherosclerosis, Coagulation Factor III is abundantly expressed in the plaque and may contribute to thrombus formation once the plaque is ruptured. Besides coagulation, it has now been recognized that Coagulation Factor III is also involved in non-hemostatic processes, such as inflammation, angiogenesis, and tumor growth and metastasis.
Gene Symbol	F3

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