Human M-CSF (CSF1) ELISA KIT (96 T)

Specifications

Product Data
Description	Human M-CSF Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human M-CSF.
Sensitivity	8pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	M-CSF, also known as CSF-1, is a four-α-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation, and differentiation. M-CSF is found as isoforms of various sizes. All isoforms contain the N-terminal 150 amino acid (aa) portion that is necessary and sufficient for interaction with the M-CSF receptor but may vary in activity and half-life. Full length human M-CSF transcripts encode a 522 aa type I transmembrane (TM) protein that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. The region of mature human M-CSF that is common to all forms shares 88%, 86%, 81%, and 74% aa sequence identity with corresponding regions of canine, bovine, mouse, and rat M-CSF, respectively.
Gene Symbol	CSF1

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