Human CD31 (PECAM1) ELISA Kit 1 x 96

Specifications

Product Data
Description	Human CD31 (PECAM1) ELISA Kit 1 x 96
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	78-5000pg/ml
Sample Type	Human serum, plasma and other biological fluids.
Sample Volume	100µl
Specificity	This kit is used for quantitative detection of PECAM-1
Sensitivity	37pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	PECAM-1 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Recombinant Human PECAM-1 Standard (250ng/ml)|0.1 mL 100x Biotin conjugated PECAM-1 Detection Antibody|0.12 mL 100x SA-HRP Conjugate|0.12 mL Assay Buffer|40 mL Sample Diluent|30 mL Wash Buffer Concentrate 20X|60 mL TMB Substrate|12 mL Stop Solution|12 mL Plate Sealer|3 pieces
Background	Platelet endothelial cell adhesion molecule (PECAM-1) also known as CD31, PCAM-1. It is a highly glycosylated transmembrane protein with a mass of approximately 130 kDa. The N-terminal domain consists of six extracellular Ig-like domains. PCAM-1 is normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. Signaling through PECAM-1 leads to the activation of neutrophils, monocytes and leukocytes. PECAM-1 is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. PECAM-1 is also expressed by many solid tumor cell lines such as hemangioma, angiosarcoma, Kaposi’s sarcoma, breast carcinoma, glioblastoma, colon carcinoma, skin carcinoma and other tumor cell lines. On the surface of these tumor cells PECAM-1 mediates the adhesion to endothelial cells. PECAM-1 modulates tumor growth by the formation of new endothelial cell tubes. Recently, it was found out that elderly patients with gastric cancer have high concentration of PECAM-1 in the serum. That suggests that the use of a serum PECAM-1 level can be a good prognostic marker. PECAM-1 contributes to at least two of the nervous system diseases, multiple sclerosis and cerebral ischaemia. High levels of soluble PECAM-1 can be used to diagnose both diseases. Increased PECAM-1 levels indicate damage in the blood brain barrier in patients with multiple sclerosis and high PECAM-1 levels can be used as a short-term prediction of a stroke in patients with cerebral ischaemia. In addition, patients with disseminated intravascular coagulation (DIC) have high levels of PECAM-1 in the serum indicating PECAM-1 as a good diagnostic marker.
Gene Symbol	PECAM1
Standard Curve	Human CD31 (PECAM1) ELISA Kit.

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