Mouse Afp ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of mouse AFP in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse AFP
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	312 pg/ml- 20,000 pg/ml
Specificity	This kit is used for quantitative detection of Mouse AFP
Sensitivity	<10pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti-mouse AFP antibody | 1 Lyophilized recombinant mouse AFP standard | 20 ng/tube x 2 Biotinylated anti-mouse AFP antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
Gene Symbol	Afp

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