Rat Angpt2 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of rat Angiopoietin-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for rat Angiopoietin-2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10000pg/ml
Specificity	This kit is used for quantitative detection of rat Angiopoietin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sensitivity	<15pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- rat Angiopoietin-2 antibody | 1 Lyophilized recombinant rat Angiopoietin-2 standard | 10ng/tube×2 Biotinylated anti- rat Angiopoietin-2 antibody | 100μl (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100μl (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Wash Buffer (25x) | 20ml Plate Sealers | 4 pieces
Background	Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Gene Symbol	Angpt2

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