Rat Tgfbi ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of rat beta IG-H3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for rat beta IG-H3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10000pg/ml
Specificity	This kit is used for quantitative detection of rat beta IG-H3
Sensitivity	<15pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- rat beta IG-H3antibody | 1 Lyophilized recombinant rat beta IG-H3 standard | 10ng/tube×2 Biotinylated anti- rat beta IG-H3 antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Gene Symbol	Tgfbi

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