Human CD47 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human CD47 in cell culture supernates and serum.
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for human CD47
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	125pg/ml-8000pg/ml
Specificity	This kit is used for quantitative detection of human CD47
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human CD47 antibody | 1 Lyophilized recombinant human CD47 standard | 10 ng/tube×2 Biotinylated anti- human CD47 antibody | 100 μl (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100 μl (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Wash Buffer (25x) | 20 ml Adhesive cover | 4
Background	CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
Gene Symbol	CD47

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