Human PEDF (SERPINF1) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human PEDF in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human PEDF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml - 10,000 pg/ml
Specificity	This kit is used for quantitative detection of Human PEDF
Sensitivity	<20pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human PEDF antibody | 1 Lyophilized recombinant human PEDF standard | 50ng/tube×2 Biotinylated anti- human PEDF antibody | 100μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Wash buffer (25x) | 20ml Adhesive cover | 4
Background	Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
Gene Symbol	SERPINF1

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