Human DKK4 ELISA KIT (1 x 96 wells)

CAT#: EA102233

For Quantitative Detection of human DKK-4 in cell culture supernates, serum and plasma(heparin, EDTA).



CNY 5,330.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description For Quantitative Detection of human DKK-4 in cell culture supernates, serum and plasma(heparin, EDTA).
Size 1 x 96 wells
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human DKK-4
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Specificity This kit is used for quantitative detection of Human DKK-4
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human DKK-4 antibody | 1
  • Lyophilized recombinant human DKK-4 standard | 10ng/tube×2
  • Biotinylated anti- human DKK-4 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.
Gene Symbol DKK4
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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