Human p53 ELISA Kit, 1 x 48-well (pre-coated)

Specifications

Product Data
Description	Human p53 ELISA Kit, 1 x 48-well (pre-coated)
Size	1 x 48 wells (pre-coated)
Format	1 x 48-well (pre-coated)
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3h45min
Signal	Colorimetric
Curve Range	3.1-100 U/ml
Sample Type	Serum, Plasma, Cell lysate
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant soluble human p53
Sensitivity	1.5 U/ml
Cross Reactivity	No cross reactivity with other human soluble molecules
Interference	No significant interference observed with available related molecules.
Components	ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background	This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
Gene Symbol	TP53
Standard Curve	Representative Standard Curve

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