VZV IgM ELISA Kit

CAT#: EA101092

VZV IgM ELISA Kit



CNY 5,510.00


货期*
9周

规格
    • 1 kit

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Specifications

Product Data
Description VZV IgM ELISA Kit
Size 1 kit
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Sample Type Cell culture supernatant, serum, plasma
Sample Volume 10 ul
Specificity Natural and recombinant human VZV IgM
Reactivity Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The Varicella Zoster Virus (VZV) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false results. Microtiter wells as a solid phase are coated with inactivated Varicella Zoster-Virus antigen (wild strain isolate). Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Varicella Zoster-Virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Varicella Zoster-Virus-specific IgM antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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