HSV-1 IgM ELISA Kit

Specifications

Product Data
Description	HSV-1 IgM ELISA Kit
Size	1 kit
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Cell culture supernatant, serum, plasma
Sample Volume	10 ul
Specificity	Natural and recombinant human HSV-1 IgM
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	The Herpes Simplex Virus Type 1 IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with recombinant gG1 protein of HSV-1. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation HSV-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of HSV-1-specific IgM antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.