AFP ELISA Kit

Specifications

Product Data
Description	AFP ELISA Kit
Size	1 kit
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0 - 160 IU/mL
Sample Type	Cell culture supernatant, serum, plasma
Sample Volume	25 ul
Specificity	Natural and recombinant human AFP
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	The AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of specimen sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti-AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of AFP in the specimen sample.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.