Human Sjögren syndrome type B antigen (SS-B)/Lupus La protein (La) IgG ELISA kit

Specifications

Product Data
Description	Human Sjögren syndrome type B antigen (SS-B)/Lupus La protein (La) IgG ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human Sjögren syndrome type B antigen (SS-B)/Lupus La protein (La) IgG
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with SSB antigen: 12x8x1 2. Sample Diluent: 1 bottle (ready to use): 22 ml 3. Calibrator: 1 Vial (ready to use): 1ml 4. Positive Control: 1 vial (ready to use): 1ml 5. Negative Control: 1 vial (ready to use): 1ml 6. Enzyme conjugate: 1 bottle (ready to use): 12ml 7. TMB Substrate: 1 bottle (ready to use): 12ml 8. Stop Solution: 1 bottle (ready to use): 12ml 9. Wash concentrate 20X: 1 bottle: 25ml
Background	Systemic autoimmune disease is characterized by the presence of circulating auto-antibodies directed to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonly referred to as Lupus is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositis/dermatomyositis. The majority can be diagnosed by clinical presentation and their antibody profiles to the various antigens involved, which include dsDNA, SM, RNP, SSA, SSB Scl-70, Jo1 and Histones. Therefore, immunoassays for autoantibodies are useful for diagnostic and prognostic evaluations of autoimmune disease. The 48 kd phosphoprotein known as SSB (La) is a transcription termination factor for RNA polymerase III. SSB shuttles between nucleus and cytoplasm and exists both free and as a component of the SSA/SSB ribonucleoprotein cytoplasmic particle. Autoantibodies to SSB are detected by ELISA in ~70-90% of primary and ~50% of secondary Sjögren syndrome as well as in ~25% of SLE and ~80% of subacute cutaneous lupus and in the majority of infants with complete heart block. SSB autoantibodies are found only in sera determined to contain SSA autoantibodies by a sensitive method; this probably reflects the association of the SS-A and SS-B antigens in a macromolecular complex.

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