Human dsDNA IgG ELISA kit

Specifications

Product Data
Description	Human dsDNA IgG ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human dsDNA IgG
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with dsDNA antigen: 12x8x1 2. Sample Diluent: 1 bottle (ready to use): 22 ml 3. Enzyme conjugate: 1 bottle (ready to use): 12ml 4. TMB Substrate: 1 bottle (ready to use): 12ml 5. Calibrator: 1 Vial (ready to use): 1ml 6. Positive Control: 1 vial (ready to use): 1ml 7. Negative Control: 1 vial (ready to use: 1ml 8. Stop Solution: 1 bottle (ready to use): 12ml 9. Wash concentrate 20X: 1 bottle: 25ml
Background	Anti-dsDNA is present in 50 % to 70% of patients with systemic lupus erythematosus (SLE). Circulating DNA/anti-DNA immune complexes are considered to play a part in the pathogenesis of SLE. The presence of anti-dsDNA is one of the diagnostic criteria for SLE. IgG antibodies to dsDNA are considered clinically most useful for the diagnosis and management of SLE. Antibodies to single stranded DNA (ssDNA) and IgM antibodies to DNA are found in a number of other connective diseases, liver diseases, as well as in some normal individuals. ELISA is the method of choice for the screening of anti-dsDNA in-patients with suspected SLE.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.