Human Cortisol ELISA kit

Specifications

Product Data
Description	Human Cortisol ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Streptavidin based ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	20-400 ng/ml
Sample Type	Serum
Sample Volume	25 µl/well
Specificity	This kit is used for quantitative detection human Cortisol
Sensitivity	20 ng/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Streptavidin coated microwells: 12x8x1 2. Cortisol Standard: 6 vials ( ready to use): 0.5 ml 3. Biotin Reagent: 1 bottle (ready to use) 4. Enzyme Conjugate (20X): 0.7 ml 5. Assay Diluent: 1 bottle (ready to use): 12 ml 6. TMB Substrate: 1 bottle (ready to use): 12 ml 7. Stop Solution: 1 bottle (ready to use): 12 ml 8. 20X Wash concentrate: 1 bottle: 25 ml
Background	Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH- dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. It has also been shown that plasma Cortisol levels elevate in response to stress. Abnormal Cortisol levels are seen with a variety of different conditions: with adrenal tumors, prostate cancer, depression, and schizophrenia. Elevated Cortisol levels and lack of diurnal variation have been identified in patients with Cushing's disease.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.