Mouse Tissue factor/F3 ELISA Kit

Specifications

Product Data
Description	Mouse Tissue factor/F3 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse Tissue factor/F3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6pg/ml-1000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and Cell lysates.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse Tissue factor
Sensitivity	<10pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-mouse F3 antibody | 1 Lyophilized recombinant mouse F3 standard | 10 ng/tube x 2 Biotinylated anti-mouse F3 antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Adhesive cover | 4 Wash Buffer (25x) | 20 ml
Background	Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Gene Symbol	F3

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