Human PON1 ELISA Kit

Specifications

Product Data
Description	Human PON1 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human PON1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates, Cell lysates, serum, plasma(heparin) and tissue homogenates.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human PON1
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human PON1 antibody 1 Lyophilized recombinant human PON1 standard 2ng/tubex2 Biotinylated anti- human PON1 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).
Gene Symbol	PON1

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