Rat EGF ELISA Kit

Specifications

Product Data
Description	Rat EGF ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Rat EGF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	7.8pg/ml-500pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of rat EGF
Sensitivity	<1pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- rat EGF antibody 1 Lyophilized recombinant rat EGF standard 10ng/tubex2 Biotinylated anti- rat EGF antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
Gene Symbol	Egf

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