Mouse MPO ELISA Kit

Specifications

Product Data
Description	Mouse MPO ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse MPO/Myeloperoxidase
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml-10,000 pg/ml
Sample Type	Cell culture supernates, Cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse MPO
Sensitivity	<10pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-mouse Mpo antibody | 1 Lyophilized recombinant mouse Mpo standard | 10ng/tube x 2 Biotinylated anti-mouse Mpo antibody | 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Gene Symbol	Mpo

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