Mouse TrkB ELISA Kit

Specifications

Product Data
Description	Mouse TrkB ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse trkB
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	93.8pg/ml-6000pg/ml
Sample Type	Cell culture supernates, Cell lysates and tissue homogenates.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse TrkB
Sensitivity	<5pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse TrkB antibody 1 Lyophilized recombinant mouse TrkB standard 10ng/tubex2 Biotinylated anti- mouse TrkB antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32—H439.
Gene Symbol	Ntrk2

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