Human Decorin ELISA Kit

Specifications

Product Data
Description	Human Decorin ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Decorin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human Decorin
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human Decorin antibody 1 Lyophilized recombinant human Decorin standard 10ng/tubex2 Biotinylated anti- human Decorin antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size.It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
Gene Symbol	DCN

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