Human Cadherin-2/N-Cadherin ELISA Kit

Specifications

Product Data
Description	Human Cadherin-2/N-Cadherin ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Cadherin-2/N-Cadherin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml - 10.000 pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human Cadherin-2
Sensitivity	< 20 pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-human CDH2 antibody | 1 Lyophilized recombinant human CDH2 standard | 50 ng/tube x 2 Biotinylated anti-human CDH2 antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Adhesive cover | 4 Wash Buffer (25x) | 20 ml
Background	Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Gene Symbol	CDH2

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.