Human TNF beta ELISA Kit

Specifications

Product Data
Description	Human TNF beta ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human TNF beta
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human TNF beta
Sensitivity	<5pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human TNF beta antibody 1 Lyophilized recombinant human TNF beta standard 10ng/tubex2 Biotinylated anti- human TNF beta antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
Gene Symbol	LTA

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