Human TRAIL ELISA Kit

Specifications

Product Data
Description	Human TRAIL ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human TRAIL
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6pg/ml-1000pg/ml
Sample Type	Cell culture supernates, Cell lysates, serum, plasma(heparin, EDTA) and saliva.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human TRAIL
Sensitivity	<1pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human TRAIL antibody 1 Lyophilized recombinant human TRAIL standard 10ng/tubex2 Biotinylated anti- human TRAIL antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.
Gene Symbol	TNFSF10

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