Rat TNF alpha ELISA Kit

Specifications

Product Data
Description	Rat TNF alpha ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Rat TNF alpha
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6 pg/ml - 1000 pg/ml
Sample Type	Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of rat TNF alpha
Sensitivity	<1pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- rat TNF alpha antibody | 1 Lyophilized recombinant rat TNF alpha standard 10ng/tube | 2 Biotinylated anti- rat TNF alpha antibody 100ul (dilution 1:100) | 1 Avidin-Biotin-Peroxidase Complex(ABC) 100ul (dilution 1:100) | 1 Sample diluent buffer 30ml | 1 Antibody diluent buffer 12ml | 1 ABC diluent buffer 12ml | 1 TMB color developing agent 10ml | 1 TMB stop solution 10ml | 1 Plate Sealers | 4 Wash Buffer (25x) 20 ml | 1
Background	Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.
Gene Symbol	Tnf

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