Human OPG ELISA Kit

Specifications

Product Data
Description	Human OPG ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human OPG
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	93.7pg/ml-6000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human OPG
Sensitivity	<5pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-human OPG antibody | 1 Lyophilized recombinant human OPG standard | 10ng/tubex2 Biotinylated anti-human OPG antibody | 100ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100ul (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4 Wash Buffer (25x) | 20ml
Background	Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
Gene Symbol	TNFRSF11B
Standard Curve	Representative standard curve for Human OPG PicoKine ELISA Kit

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