Human NGF/NGF beta ELISA Kit

CAT#: EA100327

Human NGF/NGF beta ELISA Kit



CNY 5,000.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description Human NGF/NGF beta ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human NGF/NGF beta
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates and serum.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human NGF
Sensitivity <1pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human NGF/NGF beta antibody 1
  • Lyophilized recombinant human NGF/NGF beta standard 10ng/tubex2
  • Biotinylated anti- human NGF/NGF beta antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
Gene Symbol NGF
Standard Curve
Representative standard curve for Human NGF/NGF beta PicoKine ELISA Kit
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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