Human M-CSF ELISA Kit

Specifications

Product Data
Description	Human M-CSF ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human M-CSF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Sample Type	Cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA), saliva and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human M-CSF
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human M-CSF antibody 1 Lyophilized recombinant human M-CSF standard 10ng/tubex2 Biotinylated anti- human M-CSF antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
Gene Symbol	CSF1
Standard Curve	Representative standard curve for Human M-CSF PicoKine ELISA Kit

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