Rat IL-4 ELISA Kit (96-well)

Specifications

Product Data
Description	Rat IL-4 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.02 - 1.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Rat IL-4 Ligand
Sensitivity	7pg/mL
Reactivity	Rat
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-4 (8X12) 2 vials rat IL-4 Standard lyophilized,1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-rat IL-4 antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 3 pieces Adhesive Films package insert
Background	Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils . The cDNA sequence of rat IL-4 predicts a 147 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to generate the 123 aa residue mature protein containing four potential N-linked glycosylation sites and seven cysteine residues . Mature rat IL-4 shares 60% and 44% amino acid sequence similarity with mouse and human IL-4, respectively . IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro . The biological effects of IL-4 are mediated by specific cell surface receptor complexes. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 . In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates . Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.
Gene Symbol	Il4
Standard Curve	Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.

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