Mouse IL-23 ELISA Kit (96-well)

Specifications

Product Data
Description	Mouse IL-23 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.02 - 1.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Mouse IL-23 Ligand
Sensitivity	7pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-23 (8X12) 2 vials mouse IL-23 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-mouse IL-23 antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 3 pieces Adhesive Films package insert
Background	Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12 . It is composed of two disulfide-linked subunits, a 19 kDa (p19) subunit that is unique to IL-23, and a 40 kDa (p40, IL-12) subunit that is shared with IL-12 . Mature mouse p19 and p40 share 88% and 92% aa sequence identity, respectively, with the corresponding rat subunits. IL-23 is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens including certain bacteria and viruses and/or their components . The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor-1 subunit (IL-12 R.1) and the IL-23-specific receptor subunit (IL-23 R) . IL-23 and IL-12 have overlapping and distinct biological activities. The IL-23 immune pathway induces the earliest recruitment of neutrophils to the site of infection, while the more classic host defense and cytotoxic response is stimulated by IL-12 . IL-23 has a role in the development and maintenance of a T cell subset, designated Th17, that is characterized by the production of IL-17A, IL-17F, IL-6, and TNF-.. The IL-23/IL-17 axis is an important mediator of inflammation. In mouse models, transgenic over-expression of IL-23 leads to a lethal systemic infl ammatory response . IL-23 eff ects on Th17 cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes (5, 8, 10-14). IL-23 may also play a role in increased tumor growth associated with chronic inflammation .
Gene Symbol	Il23a
Standard Curve	Representative standard curve for IL-23 ELISA. IL-23 was diluted in serial two-fold steps in Sample Diluent.

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