Mouse TGF-beta1 ELISA Kit (48-well)

Specifications

Product Data
Description	Mouse TGF-beta1 ELISA Kit (48-well)
Size	1 x 48 wells
Format	48-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	15.625-1000 pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	100 uL
Specificity	Natural and recombinant Mouse TGF-β1 Ligand
Sensitivity	15pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to mouse TGF-β1 (8 6) 2 vials mouse TGF-β1 Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-mouse TGF-β1 antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vial 1N HCl 2 vial 1.2 N NaOH/0.5 M HEPES 2 vial 2.7N NaOH/1 M HEPE 2 vial 2.5N Acetic Acid/10 M Urea 3 pieces Adhesive Films package insert
Background	Transforming Growth Factor-beta (TGF-.) is a pleiotropic cytokine which exists in five isoforms, known as TGF-.1-5. TGF-.1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. TGF-.1 is produced in very high levels by platelets. Other cellular sources of TGF-.1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-.1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-.1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-.1 inhibits the proliferation of T cells and NK cells and down-regulates the activities of activated macrophages. TGF-.1 blocks the anti-tumor activity of IL-2-bearing lymphokine-activated killer (LAK) cells. TGF-.1 has a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-.1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-.1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-.-induced regulatory T cells have been termed Ti-Treg.
Gene Symbol	Tgfb1
Standard Curve	Representative standard curve for TGF-ß1 ELISA. TGF-ß1 was diluted in serial two-fold steps in Sample Diluent.

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