Human sE-selectin ELISA Kit (48-well)
CNY 2,890.00
Specifications
Product Data | |
Description | Human sE-selectin ELISA Kit (48-well) |
Size | 1 x 48 wells |
Format | 48-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Curve Range | 780-50000pg/ml |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Human sE-selectin Ligand |
Sensitivity | 200pg/mL |
Reactivity | Human |
Interference | No significant interference observed with available related molecules. |
Background | E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1. or TNF-.) or endotoxins . Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin . Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin . The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence . E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate . Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals , probably arising from proteolytic cleavage of the surface-expressed molecule . Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions . Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent . |
Gene Symbol | SELE |
Standard Curve |
![]() Representative standard curve for sE-selectin ELISA. sE-selectin was diluted in serial two-fold steps in Sample Diluent.
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Documents
Product Manuals |