Human TSLP ELISA Kit (96-well)

Specifications

Product Data
Description	Human TSLP ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	3.9-250pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human TSLP Ligand
Sensitivity	2pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human TSLP (8X12) 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TSLP antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Thymic Stromal Lymphopoietin (TSLP) is a 23 kDa member of the IL-7 family of a-helical cytokines . It is a monomeric glycoprotein that is synthesized as a 159 amino acid (aa) precursor . TSLP appears to have species-specific functions. In mouse, TSLP was initially reported to act on NK cells, mast cells, and B cells, but this does not appear to occur in humans. In humans, TSLP is produced by a number of divergent cell types, all of which appear to target T cells, monocytes, and/or dendritic cells . On TCR-activated T cells, TSLP directly induces T cell proliferation . The significance of this direct action is unclear. On monocytes, TSLP is reported to induce the release of multiple chemokines that target CCR4, a receptor associated with the Th2 subset . TSLP is best known for its direct action on subsets of dendritic cells. In thymic medulla, HassellÂ?s corpuscle epithelium produces TSLP that acts on resident CD11c+ dendritic cells. This induces the expression of B7 family molecules on dendritic cells, which subsequently convert regional CD4+CD25+ (potentially) autoreactive T cells into CD4+CD25+FOXP3+ regulatory T cells . Allergen-challenged keratinocytes are also known to produce TSLP in skin where TSLP acts on Langerhans cells (CD1a+ immature dendritic cells) which then migrate to regional lymph nodes and express B7-2/CD86, CD83, high levels of MHC-II, and TARC. TARC attracts naive CD4+ T cells to the TSLP-activated Langerhans cells, and this interaction induces a Th2 phenotype. TSLP-induced Th2 cells are strong producers of IL-13, IL-5, and TNF-a, all of which promote allergic-type inflammations .
Gene Symbol	TSLP
Standard Curve	Representative standard curve for TSLP ELISA. TSLP was diluted in serial two-fold steps in Sample Diluent.

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