Human IL-15 ELISA Kit (96-well)

Specifications

Product Data
Description	Human IL-15 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	7.8-500pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IL-15 Ligand
Sensitivity	4pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human IL-15 (8x12) 2 vials human IL-15 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-15 antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Interleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly . IL-15 associates with IL-15 R. in the endoplasmic reticulum, and this complex is expressed on the cell surface . The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R. are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R./.c on adjacent cells . This enables cells to respond to IL-15 even if they do not express IL-15 R. . Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis . It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells . IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells . Ligation of membraneassociated IL-15/IL-15R. complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15R. expressing cells .
Gene Symbol	IL15
Standard Curve	Representative standard curve for IL-15 ELISA. IL-15 was diluted in serial two-fold steps in Sample Diluent.

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