Human IL-17 ELISA Kit (48-well)

Specifications

Product Data
Description	Human IL-17 ELISA Kit (48-well)
Size	1 x 48 wells
Format	48-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IL-17 Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human IL-17 (8x6) 1 vials human IL-17 Standard lyophilized,1000 pg/ml upon reconstitution 1 vials concentrated Biotin-Conjugate anti-human IL-17 antibody 1 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 pieces Adhesive Films package insert
Background	IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) , is produced by activated T lymphocytes, primarily by memory T cells . IL-17 appears to mediate communication between the immune system and the hematopoietic system. IL-17 is a disulfide-linked homodimer . Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence . There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins . The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri . There is at least some species specificity for in vitro action on bone-marrow stromal cells . IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-.B-mediated mechanism . IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils . IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage .
Gene Symbol	IL17A
Standard Curve	Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.

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