Human TSLP ELISA kit

Specifications

Product Data
Description	Human Interleukin-17A Elisa kit
Size	3 x 96 Well Plate
Components	Kit contents Capture Antibody: 0.5 mg/mL of mouse anti-TSLP monoclonal antibody (clone 209A2.03). Dilute in carbonate buffer pH9.6 to a working concentration of 2.5 μg /mL. Detection Antibody: 0.5mg/mL of Biotin-conjugated anti-TSLP mouse monoclonal antibody (clone 210H1.04). Dilute to a working concentration of 3μg/mL in PBS-BSA1%-Tween0.05%. Standard: Each vial contains 1μg/mL of recombinant TSLP produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended. Materials and reagents required but not provided 96well-plate Nunc Maxi Sorp Elisa coating buffer (50mM carbonate/bicarbonate buffer pH9.6) PBS-BSA1%-Tween0.05% Streptavidin-peroxydase concentrate (#SA-5004, Vector laboratories) TMB super sensitive HRP (TMBS100-0500, TEBU-BIO) Multichannel pipettes and pipette tips A standard microplate reader (620nm).
Background	TSLP (thymic stromal lymphopoïetin) is a hemopoietic protein of 159 aa (18 kDa), produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromallike cells. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is also detected in the thymus and the tissue cells of the bone marrow. TSLP affects the transition from pre-B cells in B. Alternative splicing of this gene results in two transcripts variants. TSLP is proposed to signal through a heterodimeric receptor complex composed of TSLP-receptor and IL7R chain. Expressed in many tissues, it prevents apoptosis and stimulates the growth of myeloid cells. (Reche PA et al, J Immunol., 2001, 167(1):336-43; Dorshkind. K, Nature. Immunol., 2000 (1):369-370). Monoclonal antibodies raised against human TSLP were obtained after mice immunization with TSLP-His-tagged transfected 293T cells, and were used to set up a quantitative immunoassay to determine TSLP concentration in biological samples.

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