Human HME-MMP12 ELISA kit

Specifications

Product Data
Size	3 x 96 Well Plate
Specificity	Intended use The human HME-MMP12 ELISA kit is for quantitative determination of HME-MMP12 concentrations in human cell culture supernatants and biological fluids (plasma, serum, sputum). This kit is for research purpose only. HME-MMP12 domain structures and maturation steps were described by Shapiro et al, JBC, 1993, 268, 23824-23829. The Elisa kit using the DDX284-DDX281 combination (DDXK-E-MMP12-2) will reveal: the proenzyme form (54KDa), the active form (45KDa) and the mature form (22KDa).
Components	Kit contents Capture Antibody: 0.5 mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 701E4.03). Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01). Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A8 point standard curve using 2-fold serial dilutions in sample dilution buffer, starting at a concentration of 1μg/ml is recommended.
Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is critical for invasion and destruction in pathologies such as aneurysm and emphysema. . HME mRNA and protein were previously described as expressed in human alveolar macrophages (Curci JA et al, 1998, J Clin Invest, 102(11):1900-10). The predicted molecular mass of the HME proenzyme is 54 kDa and readily undergoes NH2- and COOH-terminal processing to an active 45KDa form and a mature 22 kDa form. HME is a unique human metalloproteinase that displays elastolytic activity. We have isolated HME-MMP12 cDNA from a human CD34+-derived DC (GM-CSF+TNF) library. Expression analysis revealed HME-MMP12 expression in lung and placenta. Anti-HME-MMP12 monoclonal antibodies were obtained after mice immunization with HME-MMP12-transfected eukaryotic cells. These antibodies were used to set up a quantitative immunoassay for the detection of human HME-MMP12 (Demedts IK et al, 2006; Thorax, 61:196-201; Lapan et al, BMC Pulmonary Medicine, 2010, 10:40).

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