Human Interleukin 17A ELISA kit

Specifications

Product Data
Size	3 x 96 well Plate
Components	Kit contents Capture Antibody: 0.5 mg/mL of mouse anti-IL17 monoclonal antibody (clone 408H6.01). Dilute in 50mM carbonate buffer pH9.6 to a working concentration of 2.5 μg /mL Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-IL17 mouse monoclonal antibody (clone 405B4.01). Dilute to a working concentration of 3μg/mL in PBS-BSA1%-Tween0.05%. Standard: Each vial contains 1μg/mL of recombinant IL17 produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended. Materials and reagents required but not provided 96well-plate Nunc Maxi Sorp Carbonate buffer pH9.6 PBS-BSA1%-Tween0.05% TMB super sensitive HRP (TMBS100-0500, TEBU-BIO) Multichannel pipettes and pipette tips A standard microplate reader (620nm)
Background	IL17 (cytotoxic T lymphocyte associated antigen 8) is a CD4+T cell-derived cytokine that stimulates stromal cells and macrophages to secrete proinflammatory cytokines. IL17 regulates the activities of NF-kappa B and mitogen-activated protein kinases. hIL17 stimulates epithelial, endothelial, and fibroblastic cells to secrete cytokines such as IL6, IL8, and G-CSF and PGE2. IL17 promoted the maturation of DC progenitors, as evidenced by increased cell surface expression of costimulatory molecules and MHC class II Ag, and allostimulatory capacity. IL17 had a lesser effect on the phenotype and function of more fully differentiated myeloid DC. These findings suggest a role for IL17 in allogeneic T cell proliferation that may be mediated in part via a maturation-inducing effect on DC. IL17 appears to be a novel target for therapeutic intervention in allograft rejection. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. (Fossiez F. et al, 1996 ; J.Exp.Med, 183:2593-2603 ; Fossiez F. et al, 1998 ; Int. Rev. Immunol., 16:541-551).

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