Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Name: Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads
SKU: TA150039
Price: 6260.00

CAT＃: TA150039

Size: 500 ul 1 ml

Conjugation: Unconjugated Agarose DyLight 594 HRP Magnetic Beads

Datasheet

SDS

View other "OTI2H8 (formerly 2H8)" antibodies (7)

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CNY 6,260.00

货期*

4周

Size

Conjugation

Cited in 9 publications.

Product images

$Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# <a href='/catalog/vectors/mammalian-expression-vectors/ps100010/pcmv6-ac-gfp-mammalian-expression-vector'>PS100010</a>) or control lysate transfected with pCMV-Entry empty vector (Cat# <a href='/catalog/vectors/mammalian-expression-vectors/ps100001/pcmv6-entry-mammalian-expression-vector'>PS100001</a>). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# <a href='/catalog/antibodies/tag-antibodies/ta150043/mouse-monoclonal-tgfp-antibody-clone-oti2h8-hrp-conjugated'>TA150043</a>). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.$

Specifications

Product Data
Product Name	Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads
Clone Name	OTI2H8 (formerly 2H8)
Applications	IP, Protein Purification
Target	tGFP
Host	Mouse
Clonality	Monoclonal
Immunogen	Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell.
Formulation	PBS (pH7.4) containing 0.1% BSA, 0.02% NaN3 and 50% glycerol with final concentration 5mg/mL beads
Conjugation	Magnetic Beads
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	26 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (9)

The use of this Antibodies has been cited in the following citations:
AMPKα2 controls the anti-atherosclerotic effects of fish oils by modulating the SUMOylation of GPR120 ,null, Nature Communications ,PubMed ID 36513627 [tGFP]
De-Palmitoylation of Tissue Factor Regulates Its Activity, Phosphorylation and Cellular Functions ,null, Cancers ,PubMed ID 34359738 [tGFP]
FOSB–PCDHB13 Axis Disrupts the Microtubule Network in Non-Small Cell Lung Cancer ,null, Cancers ,PubMed ID 30658436 [tGFP]
Variants identified in PTK7 associated with neural tube defects, et al. Variants identified in PTK7 associated with neural tube defects ,null, Molecular Genetics & Genomic Medicine ,PubMed ID 30689296 [tGFP]
Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival ,Losada, A;Muñoz-Alonso, MJ;Martínez-Díez, M;Gago, F;Domínguez, JM;Martínez-Leal, JF;Galmarini, CM;, Br. J. Cancer ,PubMed ID 30420615 [TGFP]
Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release. ,null, Biochimica et biophysica acta ,PubMed ID 27599717 [tGFP]
TM4SF3 and AR: A Nuclear Complex that Stabilizes Both Proteins ,null, Molecular Endocrinology ,PubMed ID 26649804 [tGFP]
Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis ,null, Neurology: Genetics ,PubMed ID 27066543 [tGFP]
Diminished Adenosine A1 Receptor Expression in Pancreatic α-Cells May Contribute to the Pathology of Type 1 Diabetes ,null, Diabetes ,PubMed ID 24264405 [tGFP]

Documents

Product Manuals
Development Guilde Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
Antibody SDS

Resources

抗体相关资料
Product Overview Video: UltraMAB Product Overview Video: TrueMAB Webinar Recording: UltraMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Other Versions

SKU	Description	Size	Price
TA150024	Mouse monoclonal tGFP antibody (clone OTI2H8), DyLight 594 conjugated	100 ul 产品选项验证失败所有选项都是必需的	CNY 3,021.00
TA150039S	Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads	500 ul 产品选项验证失败所有选项都是必需的	CNY 3,790.00
TA150041	Mouse monoclonal turboGFP antibody, clone OTI2H8	100 ul 产品选项验证失败所有选项都是必需的	CNY 1,000.00
TA150041S	Mouse monoclonal turboGFP antibody, clone OTI2H8	30 ul 产品选项验证失败所有选项都是必需的	CNY 500.00
TA150043	Mouse monoclonal tGFP antibody (clone OTI2H8), HRP conjugated	100 ul 产品选项验证失败所有选项都是必需的	CNY 3,021.00
TA183081	Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Agarose Beads	1 ml 产品选项验证失败所有选项都是必需的	CNY 6,260.00
TA183081S	Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Agarose Beads	500 ul 产品选项验证失败所有选项都是必需的	CNY 3,790.00

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