F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Specifications

Product Data
Product Name	F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500 - 1:2,000 IHC: 1:500 - 1:2,000 ELISA: 1:5,000 - 1:20,000
Host	Donkey
Immunogen	Rat IgG whole molecule
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Note	Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Do not use sodium azide as a preservative.  Azide will substantially inhibit the enzyme activity of horseradish peroxidase.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.