F(ab')2 Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Specifications

Product Data
Product Name	F(ab')2 Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500-1:1,000 IHC: 1:250-1:500 ELISA: 1:1,000 - 1:15,000
Host	Rabbit
Immunogen	Horse IgG whole molecule
Formulation	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	0.5 mg/mL - lot specific
Conjugation	Alkaline Phosphatase
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Note	F(ab')2 Anti-Horse IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

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